THE DETERMINATION OF BLOOD UREA NITROGEN BY DIRECT NESSLERIZATION
نویسندگان
چکیده
منابع مشابه
The Determination of Blood Tjrea Nitrogen by Direct Nesslerization
The determination of urea nitrogen in blood filtrates is one of the most troublesome procedures encountered in the hospital laboratory. When the method used is that of distillation as recommended by Folin it is often necessary to repeat determinations because the solution has sucked back or frothed over. The results obtained by aeration, unless very carefully checked, are too often low and the ...
متن کاملThe Direct Colorimetric Determination of Urea in Blood and Urine
Methods for the determination of urea in biological fluids have passed through an interesting cycle (cf. Peters and Van Slyke (3) pp. 539-544). The earliest procedures were strictly chemical ones, involving precipitation of the urea as an insoluble mercury complex or decomposition of the urea by means of heat or hypobromite. The use of the highly specific enzyme urease occurring in the jack and...
متن کاملA Coulometric Determination of Urea Nitrogen in Blood and Urine.
Since the ammonia undergoes a 3-electron change, 1 mole is equal to 3 equivalents of the generated titrant. Recently we (lescril)ed an improved cud-point (letection for this system and applied it to titration of as little as 1.4 g. ammonia (2). This method has been extended to titration of ammonia formed by the action of urease on urea in blood and urine. In urine samples, ammonia may be titrat...
متن کاملBlood Urea Nitrogen
Loop diuretics are used in patients with heart failure (HF) to relieve symptoms of systemic and pulmonary venous congestion. Despite their nearly universal use for this indication, loop diuretics have not been shown to improve survival—indeed, a number of recent studies suggest an adverse relationship between the use of loop diuretics and survival (1–3). This is plausible because loop diuretics...
متن کاملThe Determination of Blood Urea
Most of the methods for the determination of urea in blood involve the use of the enzyme urease to hydrolyze the urea, since this reaction is specific. However, instead of crystalline urease (l), crude jack bean meal extracts are used. These extracts have been found to contain other enzymes besides urease, termed “ammonia-producing” enzymes, and their substrates, which may produce large quantit...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1930
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)76805-1